I needed to use my old 2013 Macbook because GCK runs only on Mac and Windows, and all my PCs are running Linux. To open this file I needed to install GCK on one of my computers. Updating macOS and getting rid of constantly nagging pop-ups gci (Illustration) files cannot be opened by SnapGene. We had used GCK until 2013 when we replaced it with the much better designed and more capable SnapGene.
#Macvector assemble plasmid how to
But how to open it? The actual GCK sequence files can be opened by the contemporary DNA construct mapping and cloning software SnapGene. And since I never delete anything, the file was still in my "Documents/Sequences/GCK/pSecTag/pSecTagI" folder. gci file for the construction of pSecTagI, which does contain a detailed description of the cloning. However, it was possible to manually create so-called Illustration files (.gci file extension), which could be used to manually document complex cloning projects and to create figures for manuscripts or presentations. GCK has had the peculiarity that it did not store the history of the construction. Last Wednesday, I spent 4 hours of my working time opening one file created with GCK version 2.5 in 1999. We were at the "cutting edge" of technology because we used a software program called Gene Construction Kit (GCK) to keep track of our clonings. In 1999, I was working in Kari Alitalo's laboratory as a Ph.D. For a new cloning project, we needed to access the plasmid maps of an old construct of mine (pSecTagN2, which was a precursor of pMosaic, aka pSecTagI) which I had composed from many different sources in 1999.
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